ABSTRACT
Para analisar cepas de Salmonella ser. Typhimurium isoladas de processos entéricos e extraintestinais humanos ocorridos no período de 1970 a 2008 de diferentes regiões do país foram selecionadas, com base nos registros contidos no banco de dados do Laboratório de Enterobactérias do IOC/FIOCRUZ, RJ, amostras do fagotipo prevalente 193, visando precipuamente o reconhecimento de clones epidêmicos. Foram selecionadas 553 cepas de Salmonella ser. Typhimurium fagotipo 193 representadas por 91, 65, 70 e 327 amostras referentes as décadas de 70, 80, 90 e ao período de 2000 a 2008, respectivamente. Na análise global da sensibilidade destas cepas, 52% apresentaram um ou mais marcadores de resistência a antibióticos incluídos no perfil ACSSuT. Este perfil de resistência completo foi verificado em 20,9% dos isolados, sendo os 21,9% restantes, sensíveis a todas as drogas testadas, especialmente no período de 2000 a 2008, representadas por 121 amostras (37,0%) em relação as 327 culturas dessa época. O maior percentual de resistência foi observado nas amostras da década de 70 (99%) sendo o perfil ACSSuT detectado em 35,2% dos isolados, ressaltando-se que todas as amostras foram isoladas de processos gastroentéricos ocorridos na cidade de São Paulo. Ao longo das quatro décadas de estudo, descreve-se um ponto de ruptura entre a prevalência de resistência e a suscetibilidade na transição entre as décadas de 80 e 90. Embora o número de isolados de Salmonalla ser. Typhimurium fagotipo 193 tenha aumentado no último período considerado, o percentual de mono e multirresistência aos antimicrobianos se situou em nível elevado (63,0%). A análise do polimorfismo obtido após macrorrestrição com a enzima Xbal revelou que cepas isoladas na década de 90 apresentaram elevado percentual de similaridade (>-85%) com cepas isoladas recentemente (período de 2000-2008), sendo agrupadas nos mesmos "subclusters". Por outro lado, as cepas da década de 70 inserem-se em "subclusters" independentes...
To analyze strains of Salmonella ser. Typhimurium isolated from human cases of enteric and extraintestinal occurred during the period 1970 to 2008 of different regions of Brazil were selected, based on records in the database from Enterobacteria Laboratory of IOC/FIOCRUZ, RJ, samples prevalent phage type 193 in order to recognition of epidemic clones. We selected 553 strains of Salmonella ser. Typhimurium phage type 193 represented by 91, 65, 70 and 327 samples concerning the 1970s, 1980s, 1990s and the period from 2000 to 2008, respectively. In a global analysis of the sensitivity of these strains, 52% had one or more antibiotic resistance markers included in the profile ACSSuT. This resistance profile was found complete in 20.9% of isolates and the remaining 21.9%, sensitive to all drugs tested, especially in the period 2000 to 2008, represented by 121 samples (37.0%) compared the 327 cultures of that time. The highest percentage of resistance was observed in the samples of the 70 (99%) being the profile ACSSuT detected in 35.2% of isolates, emphasizing that all strains were isolated from gastrointestinal processes occurring in São Paulo city. Over the four decades of study, we describe a breaking point between the prevalence of resistance and susceptibility in the transition between the 1980s and 1990s. Although the number of isolates of Salmonella ser. Typhimurium phage type 193 has increased in the last period, the percentage of mono-and-multidrug resistance to antimicrobial agents stood at high level (63.0%). The analysis of polymorphism obtained after macrorestriction with the enzyme Xbal showed that isolates in the 1990s showed a high percentage of similarity (>-85%) with strains isolated recently (2000-2008) and are grouped in the same subclusters. Moreover, the strains of the 1970s fall into subclusters independent, although the percentage of similarity between such subsclusters and the other is >- 70%, the same was observed for the strains...
Subject(s)
Humans , DNA, Bacterial/analysis , Drug Resistance, Multiple, Bacterial , Electrophoresis, Gel, Pulsed-Field/methods , Salmonella Phages/isolation & purification , Salmonella Infections/microbiology , Salmonella Infections/drug therapy , Salmonella typhimurium , Salmonella typhimurium/genetics , Salmonella typhimurium/isolation & purification , R Factors/genetics , Microbial Sensitivity TestsABSTRACT
272 isolates of Salmonella Enteritidis (111 isolated from frozen broiler chicken carcasses, 126 from human food and other biological materials involved in food poisoning outbreaks and 35 from different poultry materials) were selected for phage typing. From these, 111 were phage typed, 57.65 percent being classified as phage type 4, 32.43 percent as phage type 4a, 3.60 percent as phage type 6a and 0.90 percent as phage type 7, whereas 5.40 percent samples were not phage typeable. The predominance of phage type 4 is in agreement with the results published worldwide, and reinforces the need for studies related to the epidemiological meaning of these findings
Subject(s)
Animals , Humans , Salmonella enteritidis , Bacteriophage Typing , Brazil , Food Microbiology , Poultry Products , Salmonella enteritidis , Salmonella Food Poisoning , Salmonella PhagesABSTRACT
BACKGROUND & OBJECTIVES: Ludhiana, an industrial city of Punjab, has a large floating population where typhoid has become endemic. A retrospective study was carried out over a period of 20 years (1980-1999) at Ludhiana on the biotyping, phage typing and drug resistance pattern of Salmonella typhi. METHODS: Of a total of 1697 S. typhi isolates obtained, phage typing and biotyping were done of only 1243 isolates. Antimicrobial susceptibility pattern of these isolates was also studied. RESULTS: Of the 1243 S. typhi isolates, 963 (77.5%) and 280 (22.5%) were of biotype I and biotype II respectively. Twenty four different S. typhi phage types were prevalent in Ludhiana in the past two decades. Between 1980 and 1989, more prevalent phage types were phage type A (35%), O (17.6%) and E1 (15.1%). During 1990-1999, there was a considerable increase in the incidence of phage type E1 (48.1%). The cumulative analysis of past two decades revealed that the incidence of phage type E1 (38.8%) was most predominant. In the past one decade (1990-1999), 412 S. typhi isolates of 13 different phage types exhibited multidrug resistance (MDR) pattern ACCoT (resistant to ampicillin, chloramphenicol, co-trimoxazole and tetracycline). High chloramphenicol resistance (74.7%) and MDR pattern ACCoT (68.2%) was shown by phage type E1 of S. typhi. INTERPRETATION & CONCLUSION: An association was observed between drug resistance and phage type pattern of S. typhi as 70 per cent isolates of S. typhi phage types E1 and O exhibited ACCoT multidrug resistant pattern. Reemergence of chloramphenicol susceptibility in the last decade emphasizes the need for regular antimicrobial surveillance to minimize the misuse of these drugs.
Subject(s)
Anti-Bacterial Agents , Anti-Infective Agents/pharmacology , Drug Resistance, Microbial , Microbial Sensitivity Tests , Retrospective Studies , Salmonella Phages/classification , Salmonella typhi/drug effectsABSTRACT
A total of 51 Salmonella strains were isolated during the six month period of May 1998 till October 1998. Of these, 41 (80.3%) were Salmonella typhi, 5 (9.8%) Salmonella pararyphi A,2 (3.9%) Salmonella worthington and 1 (1.9%) Salmonella senftenberg. The prevalent phage and biotype of Salmonella typhi was E1 (75%) and type 1 (90.2%) respectively. The commonest pattern of multiple drug resistance in Salmonella typhi was ACCoT and 92.5% of these belonged to phage E1. Out of the five Salmonella paratyphi A, one belonged to phage type 1 and the others were untypable. Similarly both the strains of Salmonella typhimurium were untypable. Thus the predominant isolate was Salmonella typhi and the commonest phage and biotype were E1 and biotype1 respectively.
Subject(s)
Anti-Bacterial Agents/pharmacology , Drug Resistance, Multiple, Bacterial , Humans , Microbial Sensitivity Tests , Salmonella/classification , Salmonella Infections/epidemiology , Salmonella PhagesABSTRACT
A total of 61 bacteriophages were isolated from 100 strains of Salmonella senftenberg. Six bacteriophages were selected for typing purposes which were specific for S. senftenberg. Five phages, SasL1 to SasL5 were morphologically similar; phage SasL6 was morphologically different from the others. These phages fall into two morphological groups none of which correspond to the known tailed enterobacterial phage species. Hence, two new phage species represented by SasL1 and SasL6 are proposed.
Subject(s)
Lysogeny , Salmonella Phages/isolation & purificationABSTRACT
A total of 287 strains of S. senftenberg received from various parts of India during 1969 to 1992 were phage typed using six lysogenic phages. The typability was 90.3 per cent and 14 different phage types could be defined excluding a small group of untypable strains. A biotyping scheme was developed utilising six characters and 13 biotypes could be defined. Stern's glycerol medium proved to be the best discriminatory medium. Diversity indeces of phage typing and biotyping schemes were 0.868 and 0.503 respectively. Better discrimination was obtained when phage types were subdivided into different biotypes with a diversity index of 0.931. The schemes were found stable, reproducible and epidemiologically useful.
Subject(s)
Bacterial Typing Techniques , Bacteriophage Typing , Lysogeny , Salmonella/classification , Salmonella Phages/physiologyABSTRACT
Forty six blood culture positive cases were studied during the current outbreak of multidrug resistant typhoid fever (MRTF). The present outbreak was caused by E1 phage type and organisms were resistant to all commonly used drugs for the treatment of typhoid fever, viz., chloramphenicol (78%), co-trimoxazole (76%) and ampicillin (68%). Treatment failures with chloramphenicol (45.5%) corroborated well with in vitro resistance. No treatment failure was seen with chloramphenicol and ceftriaxone, when these drugs were used in cases infected with sensitive strains. Among the alternative drugs used in cases with in vitro sensitivity, successful clinical response was seen with ceftriaxone (4/4) and cefotaxime (8/9) as compared to cephalexin (3/5) or a combination of cephalexin and furazolidone (9/12).
Subject(s)
Ampicillin Resistance/physiology , Anti-Bacterial Agents/pharmacology , Child , Chloramphenicol Resistance/physiology , Disease Outbreaks , Drug Resistance, Microbial/physiology , Humans , India/epidemiology , Salmonella Phages/drug effects , Salmonella typhi/drug effects , Tetracycline Resistance/physiology , Trimethoprim Resistance/physiology , Trimethoprim, Sulfamethoxazole Drug Combination/pharmacology , Typhoid Fever/drug therapy , Urban PopulationABSTRACT
A total of 156 strains of Salmonella isolated at T.N. Medical College and B.Y.L. Nair Ch. Hospital, Bombay over a period of 5 years from 1983 to 1987 were subjected to Phage Typing. Out of the 111 Salmonella typhi strains, phage type A was found in highest proportion (45.95%), followed by phage type E1 (15.32%), 0(9.91%), Deg. Vi. (9.91%) and C5(5.41%). Salmonella paratyphi A had phage type pattern of 1(60.0%), 2(22.86%) and Untypable (14.29%). Majority of the Salmonella typhimurium isolates (90.0%) were untypable.
Subject(s)
Bacteriophage Typing , Humans , India , Salmonella/classification , Salmonella Infections/microbiology , Salmonella PhagesABSTRACT
Se comparó la capacidad fagocítica de células ael exudado peritoneal (CEP) de ratones CFW inmunizados con una preparación ribosomal de Salmonella typhi Ty2, con la de ratones protegidos con una vacuna de bacterias inactivadas por calor, ambas en relación con lo obtenido en animales testigo, no inmunizados. Los ribosomas se administraron subcutáneamente en una dosis inicial de 100 µg de ARN y se dio un refuerzo igual a los 14 días, ambos con adyuvantes incompleto de Freund (AIF). Los ratones inmunizados con vacuna de células muertas, recibieron una sola dosis subcutánea con 16***6 bacterias en AIF. Al cabo de 7, 11, 14, 18, 22, 25, y 31 días se indujeron y extrajeron las CEP de los animales de cada grupo e individualmente se cultivaron in vitro junto con S. Typhi Ty2 virulento no opsonizado en relación células-bacterias 1:200. La sobrevida de las bacterias fagocitadas se determinó a las 24 horas de cultivo: las CEP se romperon y por cuenta viable se enumeraron las bacterias no digeridas. Los resultados indican que las CEP de los inmunizados eliminan bacterias con mayor eficiencia que las de testigos. También se demostró que la eficiencia bactericida fue significativamente mayor (P máxima de 0.005) para las CEP de los ratones tratados con la fracción ribosomal que las CEP de los animales vacunados con bacterias intactas no viables. Fiebre tifoidea; vacuna ribosomal; inmunidad a Salmonella; Salmonella typi; fagocitosis por células peritoneales
Subject(s)
Mice , Animals , Peritoneum/cytology , Salmonella typhi/immunology , Immunization, Passive , Mexico , Mice/immunology , RNA Phages/metabolism , Salmonella Phages/immunology , Typhoid-Paratyphoid Vaccines/classificationABSTRACT
Se analizan 175 casos de fiebre tifoidea en niños de 6 meses a 15 años de edad, hospitalizados entre 1982 y 1985, intentando relacionar la gravedad de la presentación clínica con los distintos fagotipos de las S. typhi aisladas de estos enfermos. Comparadas la frecuencia e intensidad de los síntomas y signos clínicos, las alteraciones de los exámenes de laboratorio, la frecuencia de las complicaciones y de las recaídas, no se encuentran diferencias significativas entre los cuatro fagotipos predominantes (E1, 46, A, Vi degradadas). También las cepas mismas mostraron un comportamiento bioquímico y antigénico similar en el estudio de laboratorio, así como una sensibilidad uniforme a cloramfenicol (CIM 100: 4 mcg/ml)